The Manufacture Method of Potato Dextrose Agar (PDA) as Growth Media for Airborne Mold in the Science Laboratory of SMAS Pesantren IMMIM Makassar
DOI:
https://doi.org/10.53861/lomas.v5i1.472Keywords:
Potato Dextrose Agar (PDA), airborne molds, science laboratoryAbstract
Abstract
The laboratory is the main learning place apart from the classroom. Some of the places that have the highest levels of indoor air contamination, one of them is the laboratory which can be contaminated by pathogenic microorganisms including mold. This group of fungi-like mold has mycotoxins that can cause a variety of adverse health effects and pose a serious health threat when immunity decreases. There are several factors that influence the growth of mold in the laboratory, including temperature and humidity. This service aims to carry out a simple practical work on making a PDA. The method activity used is experimental based with descriptive and qualitative data analysis from practicum results. The practicum was guided directly in the science laboratory at SMAS Pesantren IMMIM Makassar and was attended by 25 students and then the students practiced in groups. Through this activity, it can be concluded that the practicum carried out can be followed by students well so that students can practice how to make PDA media which can be used as a medium for growing airborne molds.
Downloads
References
1. Sadrizadeh, S., Yao, R., Yuan, F., Awbi, H., Bahnfleth, W., Bi, Y., Cao, G., Croitoru, C., Dear, R. d., Haghighat, F., Kumar, P., Malayeri, M., Nasiri, F., Ruud, M., Sadeghian, P., Wargocki, P., Xiong, J., Yu, W., & Li, B. (2022). Indoor air quality and health in schools: A critical review for developing the roadmap for the future school environment. Journal of Building Engineering, 57, 1-23.
5. Simanjuntak, N., Khotimah, S., & Linda, R. (2015). Keanekaragaman kapang udara di ruang perkuliahan Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Tanjungpura Pontianak. Jurnal Protobiont, 4(2), 55–62.
6. Daou, R., Joubrane, K., Maroun, R. G., Khabbaz, L. R., Ismail, A., & Khoury, A. E. (2021). Mycotoxins: Factors influencing production and control strategies. AIMS Agriculture and Food, 6(1), 416-447.
7. Broto, W. (2018). Status cemaran dan upaya pengendalian aflatoksin pada komoditas serealia dan aneka kacang. Jurnal Litbang Pertanian, 37(2), 81–90.
9. Wantini, S., & Octavia, A. (2018). Perbandingan pertumbuhan jamur Aspergillus flavus pada media PDA (potato dextrose agar) dan media alternatif dari singkong (Manihot esculenta Crantz). Jurnal Analis Kesehatan, 6(2), 625.
10. Rachmatantri, I., Hadiwidodo, M., & Huboyo, H. S. (2015). Pengaruh penggunaan ventilasi (Ac dan non-ac) terhadap keberadaan mikroorganisme udara di ruang perpustakaan. Jurnal Teknik Lingkungan, 1-13.
11. Pudjadi, E., Suciyani, R., Sahira, I. G., & Pikoli, M. R. (2015). Kualitas mikrobiologis udara di salah satu pusat perbelanjaan di Jakarta Selatan. Al-Kauniyah Jurnal Biologi, 8(2), 59-65.
16. Sufiyanto, M. I., & Hefni, M. (2021). Analisis penggunaan praktikum sederhana untuk meningkatkan keterampilan proses sains Di SDN Durbuk III pamekasan tahun pelajaran 2019/2020. Eduproxima …, 3(1), 1–17.
17. Arisanti, D., Basarang, M., & Nurhidayat. (2020). Penyuluhan kesehatan perilaku hidup bersih dan sehat (PHBS) tatanan rumah tangga Desa Bajiminasa Kec. Gantarangkeke Kabupaten Bantaeng. Lontara Abdimas, 1(1), 38-42.
Book:
3. Wasilah, S. Z., Nasution, J., Rahmiati, Fadillah, M. A., Bangu, Supriyanto, Salim, M., Darsono, K., Anwar, A. Y., Asikin, Z. F., Nurhayati, E., & Malik, N. (2023). Mikologi. Jawa Tengah: Eureka Media Aksara.
4. Apriliawati, A. (2009). Ensiklopedia IPA. Jakarta: PT Lentera Abadi.
8. Cappuccino, J. G., & Sherman, N. (2014). Manual Laboratorium Mikrobiologi. Jakarta: EGC.
12. Purnama, D. I. (2017). Analisis Kualitas Fisik Udara dan Keberadaan Jamur di Ruang Paviliun RSD dr. Soebandi Jember. Skripsi. Jember: Bagian Kesehatan Lingkungan dan Kesehatan Keselamatan Kerja Fakultas Kesehatan Masyarakat Universitas Jember.
13. Hafsan. (2014). Mikrobiologi analitik. Makassar: Alauddin University Press.
14. Umrati, & Wijaya, H. (2020). Analisis data kualitatif teori konsep dalam penelitian pendidikan. Makassar: Sekolah Tinggi Theologia Jaffray.
15. Wijaya, H. (2018). Analisis data kualitatif ilmu pendidikan teologi. Makassar: Sekolah Tinggi Theologia Jaffray.
Internet Sources:
2. Sela Septi Dwi Arista. (2023). Dampak Pencemaran Mikroba di Udara bagi Kesehatan Tubuh. Surabaya, Jawa Timur: Unair News. Retrieved Mei 7, 2023, from https:// https://unair.ac.id/dampak-pencemaran-mikroba-di-udara-bagi-kesehatan-tubuh/
